Phylogenetic Construction of Stingless Bees in Bandar Lampung based on 16S rRNA Gene

Priyambodo Priyambodo , Elly Lestari Rustiati , Dian Neli Pratiwi , Alvin Wiwiet Susanto , Eko Agus Srihanto , Enny Saswiyanti

Abstract


Stingless bees are one of the honey-producing groups that are currently in increasing demand. The significant rise in honey demand necessitates research into the variety of stingless bee species that have the potential to produce high-quality honey in large quantities. This study aims to determine the genetic relationship of stingless bees from two colonies in Rajabasa, Bandar Lampung. Samples were collected from two different colonies in Rajabasa, Bandar Lampung. Molecular analysis was conducted, including DNA extraction, amplification, DNA sequencing, and phylogenetic analysis using the 16S rRNA gene. The study assessed homology values, genetic distance, and the phylogenetic map. The results indicated that the individual samples, GB1 and GB2, found in Bandar Lampung, were closely related to the species Heterotrigona itama. This was confirmed by the query value of both samples being 100%, with similarity values of 96.99% and 97.20%, respectively. The genetic distance analysis, visualized in the phylogenetic map, also shows that the two samples are close relatives of Heterotrigona itama.

ABSTRAK: Lebah tanpa sengat menjadi salah satu kelompok penghasil madu yang saat ini makin banyak diminati masyarakat. Peningkatan kebutuhan madu yang signifikan, memerlukan penelitian tentang ragam spesies lebah tanpa sengat yang potensial dalam menghasilkan madu yang berkualitas prima dalam jumlah yang melimpah. Penelitian ini telah dilakukan dengan tujuan untuk mengetahui kedekatan genetik lebah tanpa sengat dari dua koloni di Rajabasa, Bandar Lampung. Sampel diambil dari dua koloni yang berbeda di Rajabasa, Bandar Lampung. Analisis molekuler telah dilakukan dengan tahapan ekstraksi DNA, amplifikasi DNA, sekuensing DNA, dan dilanjutkan dengan analisis filogenetik dengan menggunakan gen 16S rRNA. Analisis dilakukan untuk mengetahui nilai homologi, jarak genetik, dan peta filogenetik. Hasil menunjukkan bahwa individu sampel GB1 dan GB2 yang ditemukan di Bandar Lampung mempunyai kedekatan dengan spesies Heterotrigona itama. Hal ini ditunjukkan dengan nilai query keduanya sebesar 100% dan nilai similarity masing-masing adalah 96,99% dan 97,20%. Analisis jarak geneti yang divisualisasi dalam peta filogenetik juga menunjukkan bahwa kedua sampel yang diperoleh merupakan kerabat dekat dari Heterotrigona itama.


Keywords


16S rRNA gene; Bandar Lampung; stingless bee.

Full Text:

PDF

References


Ahmed, A., Tul-Noor, Z., Lee, D., Bajwah, S., Ahmed, Z., Zafar, S., Syeda, M., Jamil, F., Qureshi, F., Zia, F., Baig, R., Ahmed, S., Tayyiba, M., Ahmad, S., Ramdath, D., Tsao, R., Cui, S., Kendall, C. W. C., De Souza, R. J., & Sievenpiper, J. L. (2023). Effect of honey on cardiometabolic risk factors: a systematic review and meta-analysis. Nutrition Reviews, 81(7), 758–774. https://doi.org/10.1093/nutrit/nuac086

Bunjkar, A., Walia, P., & Sandal, S. S. (2024). Unlocking Genetic Diversity and Germplasm Characterization with Molecular Markers: Strategies for Crop Improvement. Journal of Advances in Biology & Biotechnology, 27(6), 160–173. https://doi.org/10.9734/jabb/2024/v27i6873

Cabezas-Mera, F., Cedeño-Pinargote, A. C., Tejera, E., Álvarez-Suarez, J. M., & Machado, A. (2024). Antimicrobial activity of stingless bee honey (Tribe: Meliponini) on clinical and foodborne pathogens: A systematic review and meta-analysis. Food Frontiers, 5(3), 964–993. https://doi.org/10.1002/fft2.386

Chika, S., & Zahro, S. (2024). An In Silico Approach for Evaluation of ITS, rbcL, and psbA-trnH for DNA Barcoding of Eugenia spp. Biosfer: Jurnal Tadris Biologi, 15(1), 153. https://doi.org/10.24042/biosfer.v15i1.21064

Fajari, M. F., Budhi, G., & Umezaki, M. (2023). A mini-ethnography of honey gathering: The practice and its contribution to livelihood systems in rural areas. ETNOSIA : Jurnal Etnografi Indonesia, 8(2), 212–229. https://doi.org/10.31947/etnosia.v8i2.28131

Fatwa, E. B., Yoswaty, D., & Effendi, I. (2021). Identification of Indigenous Bacteria from Dumai Sea Waters Using 16S rRNA Method. Journal of Coastal and Ocean Sciences, 2(3), 184–188. https://doi.org/10.31258/jocos.2.3.184-188

Godiyal, S., Kumar, A., Kumar, V., Uniyal, A., Gupta, S., Kumar, V., & Allen, S. (2024). A Review On Threatened Medicinal Plants In Uttarakhand And Their Genetic Diversity Assessment Through Molecular Markers. Journal of Advanced Zoology, 45(3)68-79.

Harianja, A. H., Adalina, Y., Pasaribu, G., Winarni, I., Maharani, R., Fernandes, A., Saragih, G. S., Fauzi, R., Tampubolon, A. P., Njurumana, G. N., Sukito, A., Aswandi, A., Kholibrina, C. R., Siswadi, S., Kurniawan, H., Hidayat, M. Y., Wahyuni, R., Koeslulat, E. E., Heryanto, R. B., & Kuspradini, H. (2023). Potential of Beekeeping to Support the Livelihood, Economy, Society, and Environment of Indonesia. Forests, 14(2), 1–37. https://doi.org/10.3390/f14020321

Iskandar, A. U., Ethica, S. N., Sukeksi, A., Mukaromah, A. H., Sulistyaningtyas, A. R., & Darmawati, S. (2021). Molecular systematic and phylogenetic analysis of indigenous bacterial isolates with potential as bioremediation agent based on 16S rRNA gene analysis. IOP Conference Series: Earth and Environmental Science, 743(1). https://doi.org/10.1088/1755-1315/743/1/012010

Joaty, J. Y., Rahman, M. M., Amin, M. R., Khan, M. A. R., & Hassan, J. (2022). Mitochondrial Dna Based Phylogeny And Haplotype Networking Of European Honeybee Apis Mellifera L. In Bangladesh. Serangga, 27(3), 220–237.

Kadarsah, A., Putra, A. P., Nurliani, A., Suhartono, E., & Ibrahim, S. (2024). Morphological Characteristics and Nest Structure of Stingless Bee (Heterotrigona itama) from Different Meliponiculture Practices. Biosaintifika: Journal of Biology & Biology Education, 15(1), 49–62. https://doi.org/10.15294/biosaintifika.v15i1.3503

Kamarudin, K. R., Rehan, A. M., Hashim, R., Usup, G., & Rehan, M. M. (2016). Phylogenetic relationships within the genus holothuria inferred from 16S mitochondiral rRNA gene sequences. Sains Malaysiana, 45(7), 1079–1087.

Lestari, D. (2022). Pengukuran Kesadaran Konsumen Terhadap Merek Madu Di Pt Suhita Lebah Indonesia. Thesis. Politeknik Negeri Lampung.

Mas’ud, A., Hasan, S., & Sundari, S. (2023). Identifikasi Jenis Lebah Madu Asal Kepulauan Sula Menggunakan Aplikasi Dna Barcode Lco Gen. Jurnal Biosilampari : Jurnal Biologi, 5(2), 163–168. https://doi.org/10.31540/biosilampari.v5i2.1888

Melia, S., Juliyarsi, I., Kurnia, Y. F., Aritonang, S. N., Rusdimansyah, R., Sukma, A., Setiawan, R. D., Pratama, Y. E., & Supandil, D. (2024). Profile of stingless bee honey and microbiota produced in West Sumatra, Indonesia, by several species (Apidae, Meliponinae). Veterinary World, 17(4), 785–795. https://doi.org/10.14202/vetworld.2024.785-795

Muthmainnah, R., Mulyani, L. S., & Fitriyani, W. (2023). Nature Club Activity: Knowledge Exploration on Javan Slow Loris (Nycticebus javanicus) and Protected Endemic Animals in Conservation Education Program. Biosfer: Jurnal Tadris Biologi, 14(1), 131–139. https://doi.org/10.24042/biosfer.v14i1.17766

Pippinato, L., Blanc, S., Mancuso, T., & Brun, F. (2020). A sustainable niche market: How does honey behave? Sustainability (Switzerland), 12(24), 1–14. https://doi.org/10.3390/su122410678

Priyambodo, P., Rustiati, E. L., Pratiwi, D. N., Susanto, A. W., Imtitsal, A., Fahrezi, A., Febriansyah, M., Kusuma, A. W., Srihanto, E. A., Saswiyanti, E., Sidik, M., Sa’uddah, L. D., Lestari, I. A., Yani, A. A., & Ramadhan, V. (2023). Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran. Jurnal Biologi Tropis, 23(1), 557–563. https://doi.org/10.29303/jbt.v23i1.6470

Purwanto, H., Soesilohadi, R. C. H., & Trianto, M. (2022). Stingless bees from meliponiculture in South Kalimantan, Indonesia. Biodiversitas, 23(3), 1254–1266. https://doi.org/10.13057/biodiv/d230309

Qamar, W., & Rehman, M. (2020). rief History and Traditional Uses of Honey. Therapeutic Applications of Honey and Its Phytochemicals, 1(1), 1–10. https://doi.org/https://doi.org/10.1007/978-981-15-6799-5_1

Rachmawati, R. D., Agus, A., Umami, N., Agussalim, & Purwanto, H. (2022). Diversity, distribution, and nest characteristics of stingless bees (Hymenoptera: Meliponini) in Baluran National Park, East Java, Indonesia. Biodiversitas, 23(8), 3890–3901. https://doi.org/10.13057/biodiv/d230805

Rahmad, B., Damiri, N., Hanafiah, Z., Adriani, D., & Hanum, L. (2024). Food source diversity and honey production in stingless bee meliponiculture, Ogan Komering Ulu Timur, South Sumatra, Indonesia. Biodiversitas, 25(6), 2747–2756. https://doi.org/10.13057/biodiv/d250645

Remero, C., Sosa, N., Vallejos, O., Navarro, A., Yamul, D., & Baldi, C. (2024). Physicochemical, microbiological, and sensory properties of stingless bee honey from Argentina. Journal of Apicultural Research, 1(1), 1–12. https://doi.org/https://doi.org/10.1080/00218839.2024.2350310

Rusdimansyah, R., Khasrad, K., Jaswandi, J., Rusfidra, R., & Aulia, D. (2024). Heterotrigona itama workers bees homing ability as the basis for colony placement. Biodiversitas, 25(6), 2478–2483. https://doi.org/10.13057/biodiv/d250616

Setiawan, R. D., Melia, S., Juliyarsi, I., & Rusdimansyah. (2024). Investigation of Stingless Bee Honey from West Sumatra as an Antihyperglycemic Food. Preventive Nutrition and Food Science, 29(2), 170–177. https://doi.org/10.3746/pnf.2024.29.2.170

Siti Nurfalinda, Fadila Sirwati, & Linda Advinda. (2023). Bio Sains : Jurnal Ilmiah Biologi. Bio Sains Jurnal Ilmiah Biologi, 2(2), 62–66.

Sriwahyuni, D., Suwarno, S., Rizki, A., & Siregar, Z. (2023). Morphometrics of Heterotrigona Itama Nest Components in the Pocut Meurah Intan Grand Forest Park, Aceh Besar Regency, Indonesia. Jurnal Penelitian Pendidikan IPA, 9(11), 10475–10483. https://doi.org/10.29303/jppipa.v9i11.5216

Suhandy, D., Al Riza, D. F., Yulia, M., & Kusumiyati, K. (2023). Non-Targeted Detection and Quantification of Food Adulteration of High-Quality Stingless Bee Honey (SBH) via a Portable LED-Based Fluorescence Spectroscopy. Foods, 12(16). https://doi.org/10.3390/foods12163067

Tarlinton, B., Massaro, F. C., & Hauxwell, C. (2023). 16S Amplicon Metabarcoding of the Nest Materials of Native Australian Stingless Bees. Microbiology Resource Announcements, 12(4), 16–19. https://doi.org/10.1128/mra.01181-22

Tola, Y. H., Waweru, J. W., Ndungu, N. N., Nkoba, K., Slippers, B., & Paredes, J. C. (2021). Loss and gain of gut bacterial phylotype symbionts in afrotropical stingless bee species (Apidae: Meliponinae). Microorganisms, 9(12), 20–23. https://doi.org/10.3390/microorganisms9122420

Trianto, M., Arisuryanti, T., Purwanto, H., & Ubaidillah, R. (2023). Updated Species Check-list of the Indonesian Stingless Bees (Hymenoptera, Apidae, Apinae, Meliponini). Journal of Tropical Biodiversity and Biotechnology, 8(2). https://doi.org/10.22146/jtbb.77160

Trianto, M., & Purwanto, H. (2020a). Molecular Phylogeny of Stingless Bees in the Special Region of Yogyakarta Revealed Using Partial 16S rRNA Mitochondrial Gene. Buletin Peternakan, 44(4), 186–193. https://doi.org/10.21059/buletinpeternak.v44i4.55539

Trianto, M., & Purwanto, H. (2020b). Morphological characteristics and morphometrics of stingless bees (Hymenoptera: Meliponini) in Yogyakarta, Indonesia. Biodiversitas, 21(6), 2619–2628. https://doi.org/10.13057/biodiv/d210633

Zafar, M., Latafat, T., Zehra, A., & Farooqui, Y. (2020). Therapeutic properties of honey: a review of literature. Res. Rev. A J. Pharmacol, 10(1), 41–49.




DOI: http://dx.doi.org/10.24042/biosfer.v15i2.24589

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2024 Biosfer: Jurnal Tadris Biologi

License URL: https://creativecommons.org/licenses/by-sa/4.0